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Wednesday, July 29, 2020 | History

3 edition of Macrophage heterogeneity established by immunocytochemistry found in the catalog.

Macrophage heterogeneity established by immunocytochemistry

Christine D. Dijkstra

Macrophage heterogeneity established by immunocytochemistry

by Christine D. Dijkstra

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  • 24 Currently reading

Published by Gustav Fischer in Stuttgart, New York .
Written in English

    Subjects:
  • Macrophages.

  • Edition Notes

    Includes bibliographical references (p. 56-65) and index.

    StatementChristine D. Dijkstra, Jan G.M.C. Damoiseaux.
    SeriesProgress in histochemistry and cytochemistry -- v. 27, no. 2.
    ContributionsDamoiseaux, Jan G. M. C.
    The Physical Object
    Paginationvi, 65 p. :
    Number of Pages65
    ID Numbers
    Open LibraryOL18140149M
    ISBN 103437115138

    The results of this study and the new markers we have identified, including a new CD38/Egr2-based flow cytometry strategy to distinguish M1 and M2 macrophages, can be found in Jablonski et al. Immunocytochemistry confirmed enhanced expression of the proinflammatory marker CD14 in M-Mac relative to GM-Mac. Phenotypical heterogeneity of macrophages is a result of a Established.

    Microglia and infiltrating macrophages are thought to orchestrate the central nervous system (CNS) response to injury; however, the similarities between these cells make it challenging to distinguish their relative contributions. We genetically labeled microglia and CNS-associated macrophages to distinguish them from infiltrating macrophages. Using single-cell RNA sequencing, we describe. Cardiovascular disease (CVD) is a global epidemic, currently representing the worldwide leading cause of morbidity and mortality. Atherosclerosis is the fundamental pathophysiologic component of CVD, where the immune system plays an essential role. Monocytes and macrophages are key mediators in this aspect: due to their heterogeneity and plasticity, these cells may act as either pro- or anti.

    Macrophages are present in all stages of atherosclerosis and are considered fundamental to atherogenesis and the behavior of established plaques. 1 Lipid-laden foam cells are derived from circulating monocytes that migrate into the vessel wall, 2,3 and inhibition of monocyte migration, for example by disrupting a variety of chemokine/chemokine receptors, inhibits atherosclerosis . Dijkstra, C.D. & Damoiseaux, J.G. () Macrophage heterogeneity established by immunocytochemistry. Prog Histochem Cytochem. 27 (2): Whiteland, J.L. et al. () Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. J Histochem Cytochem. 43 (3):


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Macrophage heterogeneity established by immunocytochemistry by Christine D. Dijkstra Download PDF EPUB FB2

Macrophage Heterogeneity Established by Immunocytochemistiry Author links open overlay panel Christine D. Dijrstra MD, PhD Jan G.M.C. Damoiseaux PhD * Show moreCited by: Get this from a library. Macrophage heterogeneity established by immunocytochemistry.

[Christine D Dijkstra; Jan G M C Damoiseaux]. Macrophage heterogeneity established by immunocytochemistry. (PMID) Abstract Citations; Related Articles; Data; BioEntities; External Links ' ' Dijkstra CD, ' ' Damoiseaux JG Progress in Histochemistry and Cytochemistry [01 Jan27(2)] Type: Cited by: Book review Full text access Macrophage heterogeneity established by immunocytochemistry, Christine D.

Dijkstra, Jan G.M.C. Damoiseaux. Gustav Fischer Verlag, Stuttgart — Jena — New York (), 66 pages with 16 figures and 4 tables. Soft cover, DM 80,- (pref. price for subscribers to the series DM 72,-). They highlight the interaction between macrophages and T cells, along with immune response gene control and macrophage secretion of a number of lymphostimulatory molecules.

Organized into six sections encompassing 35 chapters, this volume begins with an overview of antigen handling and presentation, immune response gene control, antigen-presenting cells, and factors affecting lymphocyte-macrophage Edition: 1.

Definition of macrophages in bone marrow and spleen, suggested novel cell adhesive interactions and new monoclonal antibodies were subsequently isolated to discover some of the surface molecules involved; reagents for antigens such as CD 25 exemplified and clarified the striking heterogeneity between marginal metallophils, for example, and red pulp macrophages in mouse spleen.

Macrophage heterogeneity can be identified in situ by differentiation antigens, fate mapping and gene expression patterns Traditionally, the identification of macrophages in tissues depended on morphology, histologic staining and intravital labelling with phagocytic particles.

The ability to assess the responses of cells exposed to nanoparticles at the single cell level will lead to mechanistic understanding of their toxicity and safety.

Here, we demonstrated the heterogeneity of cell uptake and cellular responses in a QDots-exposed macrophage using a high-content screening (HCS) analysis. The dynamic uptake and intracellular location of QDots were characterized by. Using immunocytochemistry and flow cytometry methods we reveal a striking heterogeneity in macrophage apoE expression in both cell types.

In phorbol-ester-differentiated THP-1 macrophages, 5% of the cells over-expressed apoE at levels more than fold higher than the rest of the population. Abstract | Heterogeneity of the macrophage lineage has long been recognized and, in part, is a result of the specialization of tissue macrophages in particular microenvironments.

Circulating monocytes give rise to mature macrophages and are also heterogeneous themselves, although the physiological relevance of this is not completely understood. Tumor-associated macrophages (TAMs), one of the most abundant immune components in gastric cancer (GC), are difficult to characterize due to their heterogeneity.

Macrophages play a central role in tuberculosis, as the site of primary infection, inducers and effectors of inflammation, innate and adaptive immunity, as well as mediators of tissue destruction and repair. Early descriptions by pathologists have emphasized their morphological heterogeneity in granulomas, followed by delineation of T lymphocyte-dependent activation of anti-mycobacterial.

Part of the Human Cell Culture book series (HUCC, volume 4) Naito M () Macrophage heterogeneity in development and differentiation Arch Histol Cytol PubMed Google Scholar. Dijkstra CD and Damoiseaux JG () Macrophage heterogeneity established by immunocytochemistry Prog Histochem Cytochem 1– PubMed Google Scholar.

Immunocytochemical analysis of antigen markers established that macrophages can be distinguished from other cell types in situ; particularly striking was the discovery that resident macrophages in different organs expressed tissue-specific signatures as.

Part of the Results and Problems in Cell Differentiation book series (RESULTS the presence of distinct macrophage populations within the same microenvironment indicates that macrophage heterogeneity may also be influenced outside of tissue specialization.

The F4/80 molecule was established as a unique marker of murine macrophages when a. Sandor et al. have described the heterogeneity of granuloma formation, i.e., Th1-type cytokines including IFN-γ induce classical activation of macrophages in type 1 granulomas (as in tuberculosis and sarcoidosis) and Th2-type cytokines such as IL-4 and IL induce alternative activation of macrophages in type 2 granulomas (Sandor et al.

Tissue macrophages: heterogeneity and functions Siamon Gordon1,2* and Annette Plüddemann3 Abstract Macrophages are present in all vertebrate tissues, from mid-gestation throughout life, constituting a widely dispersed organ system.

They promote homeostasis by responding to internal and external changes within the body, not only as phagocytes in. In reality, macrophage phenotype is a product of the complex microenvironment in which the cell resides and is not necessarily a static or terminal state.

Macrophage characteristics, therefore, vary from tissue to tissue and this is an essential concept in our understanding of the ‘heterogeneity’ for which macrophages are (in)famous.

However, cross-tissue heterogeneity of tissue-resident macrophages has not been well characterized. We merged single-cell transcriptome data of macrophages from diverse tissues to search for subclasses. We found that macrophages can be classified into 13 subtypes (Figures 6D and 6E; Table S6).

They show preferential expression of different C. Dijkstra CD, Damoiseaux JGMC: Macrophage heterogeneity established by immunocytochemistry.

Progr Histochem Cytochem.CAS Google Scholar. Macrophage polarization is increasingly recognised as an important pathogenetic factor in inflammatory and neoplastic diseases.

Proinflammatory M1 macrophages promote T helper (Th) 1 responses and show tumoricidal activity. M2 macrophages contribute to tissue repair and promote Th2 responses. CD68 and CD are used to identify macrophages in tissue sections.Tissue-resident macrophages play critical roles in controlling homeostasis, tissue repair, and immunity.

Inflammatory macrophages can sustain tissue damage and promote the development of fibrosis during infections and sterile tissue injury. The NLRP3 inflammasome and its effector cytokine IL-1β have been identified as important mediators of fibrosis.

Epirubicin, an anthracycline. Heterogeneity of the macrophage lineage has long been recognized and, in part, is a result of the specialization of tissue macrophages in particular microenvironments.